About Fura-2 (Ca2+free)

Fura-2, AM is a cell-permeant ratiometric calcium indicator. When Fura-2, AM loads a cell and is intracellularly hydrolyzed, it exhibits an increase in excitation efficiency at 336nm but not at 368nm. This increases the emission intensity monitored at ~510nm when bound to calcium while excited by 336nm energy while the reduced intensity can be monitored at the ~510nm emission off the 368nm excitation. Because of the unique spectral characteristics, this probe is most commonly used in fluorescence microscopy.